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JoVE Video Journal Publication: Quantification of Humic and Fulvic Acids

Dr. Richard T. Lamar and Dr. Hiarhi Monda of our Humic Research Laboratory, with assistance from analytical chemist Ryan Fountain, have published a methodology video in the biochemistry section of the peer-reviewed online video journal, JoVE.

The video, Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products, shows the step-by-step laboratory methodology (the New Standard Method) for gravimetric quantification of humic substances (e.g., humic and fulvic acids) on an ash-free basis, in dry and liquid materials from soft coals (i.e., oxidized and non-oxidized lignite and sub-bituminous coal), humate ores and shales, peats, composts and commercial fertilizers and soil amendments.

In the video introduction, Dr. Lamar states, “The New Standard Method for quantification of humic acids provides a more accurate and precise analysis compared to the existing regulatorily accepted methods, and it also provides a standard method for pure hydrophobic fulvic acid quantification. The advantage of this protocol is that it provides a gravimetric analysis of humic and hydrophobic fulvic acid concentrations on an ash-free basis, and the extraction process has been optimized to obtain the highest recoveries of both humic and fulvic acids from samples.

At the video’s conclusion, Dr. Monda states, “Following this procedure, the dry humic and fulvic acids obtained can be used for characterization purposes, such as the carbon-13 and the proton NMR electron resonance, and the ultrahigh resolution mass spectrometry, among other useful techniques. This can be used for characterization of the humus chemistry, as well as being a useful tool to dig deep into the structure-activity relationship with plant fitness and the underlying plant defense mechanisms.

Direct link to video on the JoVE Website: https://www.jove.com/v/61233/quantification-humic-fulvic-acids-humate-ores-doc-humified-materials (A free subscription will be required to view the entire video on the JoVE Website.)

From the JoVE Website: Filmed at the world’s top scientific institutions, JoVE videos bring to life the intricate details of cutting-edge experiments enabling efficient learning and replication of new research methods and technologies. JoVE is a peer-reviewed scientific video journal that is indexed in PubMed and Web of Science.

Use of Biostimulants and Buffers for Upset Recovery in Paper Mill Wastewater Systems

By Heather Jennings, PE

Industrial pulp and paper wastewater is considered one of the more challenging waters to treat using biological methods, which depend on microbial activity to effectively remediate the wastewater.

Wastewater treatment systems are often influenced/impacted by increased hydraulic and/or COD (chemical oxygen demand) loading as mills add new chemicals or otherwise modify mill operations. These events oftentimes inhibit the wastewater microbial activity, causing “upsets” and, potentially, discharge-limit violations. However, providing the necessary biostimulants and buffers to the microbial system—as we describe in this case study from a paper mill in China—can significantly improve system-upset recovery time and overall operational stability. Continue Reading

The Impact of Commonly Abused and Illicit Drugs in Wastewater Treatment

By Heather Jennings, PE, Senior Project Engineer for Probiotic Solutions®

I was attending a wastewater conference and overheard an operator talking about how a drug bust turned his lagoon orange and almost put him out of compliance with his permit. At another location, I was told that the city I was visiting had been hit by an unexpected source of ammonia that almost “wiped out” their bugs. I asked an operator if it was possible that the influent to his wastewater system might have illicit drugs in it. His reply was to the effect that, although he frequently found drug paraphernalia in his screens, he didn’t know of any illicit drugs being present in his system. So, I started wondering what illicit drug impacts really have on wastewater systems. The following is what I found. Continue reading

Use of Biostimulants & Buffers for Upset Recovery at Paper Mill Wastewater System

Industrial Water & Wastes Digest has published a Probiotic Solutions® article, “Use of Biostimulants & Buffers for Upset Recovery at Paper Mill Wastewater System.”

Two specific Probiotic Solutions liquid bioremediation products were used to address process upsets at a paper mill in China. The products involved were Bio Energizer (BE)—a scientific formulation of organic acids, buffers, natural biological stimulants, micronutrients and energy systems—and Micatrol (MT), a specialized product that uses organic acid as a substrate to buffer wastewater microbial life. Continue reading

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